The breakage of DNA molecule at desired places to
isolate a specific DNA segment and then insert it in another
DNA molecule at a desired position to obtain recombinant
DNA. The technique often called genetic engineering is
useful as genes can be isolated, cloned and characterised so
that the technique has led to significant progress in all areas
of molecular biology. Using the recombinant DNA
technology we can isolate and clone single copy of gene or
a DNA segement into indefinite number of copies, all
identical. This has become possible because bacteriophages
and plasmids reproduce in their usual style even after
insertion of foreign DNA so that the inserted DNA will also
replicate faithfully with the parent DNA. This technique is
called ‘gene cloning’.
isolate a specific DNA segment and then insert it in another
DNA molecule at a desired position to obtain recombinant
DNA. The technique often called genetic engineering is
useful as genes can be isolated, cloned and characterised so
that the technique has led to significant progress in all areas
of molecular biology. Using the recombinant DNA
technology we can isolate and clone single copy of gene or
a DNA segement into indefinite number of copies, all
identical. This has become possible because bacteriophages
and plasmids reproduce in their usual style even after
insertion of foreign DNA so that the inserted DNA will also
replicate faithfully with the parent DNA. This technique is
called ‘gene cloning’.
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